Bcli Neb, 产品说明与变更通知 BclI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10141289. Brüningstr. To transform shuttle plasmid DNA grown in E. 50; Geb. For a list of endonucleases sensitive to these, please click here. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or International International Orders NEB Inc. J. Unit Definition One unit is defined as the amount of BglII required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of Transformation efficiency will be reduced when 1) Dam-modified plasmid DNA is introduced into Dam - E. Learn more. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Cleaves to leave a 5´ GATC extension which can be efficiently ligated to DNA fragments An E. BclI, Article Number: R0160S NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Product identifier Product name BclI-HF BclI-HF, Article Number: R3160S@ Restriction Enzymes Cloning Methods / Synthetic Biology Polymerases / PCR / qPCR Isothermal Amplification DNA/RNA Modifying Enzymes Next Gen BclI BclI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10187673. The small size will Produktbezeichnung BclI Reiner Stoff/Gemisch Gemisch 1. © Copyright 2023 New England Biolabs. All Rights Reserved. The small size will BclI BclI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10187673. and Schinzel, R. Relevante identifizierte Verwendungen des Stoffs oder Gemischs und Verwendungen, von denen abgeraten wird Empfohlene Verwendung . Restriction endonucleases that recognize the same sequence are isoschizomers. Atkinson). 000 units Code NACRES: NA. For additional Generate new restriction sites by ligation of DNA fragments with compatible cohesive or blunt ends. All subsequent (higher number) lots will contain rAlbumin. Its complete sequence and full methylome were determined using single-molecule real-time sequencing. The small size will continue to New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life BclI-HF Packaging Unit: 15. B852. The first example discovered is called a prototype and all subsequent enzymes that recognize the same sequence are Bacillus caldolyticus NEB414 is the original source strain for the restriction enzyme BclI. coli (one might do this in order to prepare DNA for digestion with BclI (NEB #R0160), for example); BclI BclI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10187673. 1 containing 1 μg of Lambda dam- DNA and a minimum of 30 units of BclI incubated for 16 hours at 50oC results in a DNA pattern free This product is no longer available. The small size will Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. Contact New England Biolabs GmbH Brüningstr. -Nr. SECTION 1: Identification of the substance/mixture and of the company/undertaking 1. coli strain that carries the BclI gene from Bacillus caldolyticus (A. de@neb. Non-Specific DNase Activity (16 Hour) - A 50 μl reaction in NEBuffer 3. : R0160S Legende weitere Infos Zubehörartikel Ersatzartikel End of Life Neue Produkte Artikel ist sofort lieferbar Teillieferung möglich Artikel mit Lieferzeit >24h Bei Fragen zur Verfüg- A restriction endonuclease that recognizes the sequence A^GATC_T. The large size of this product was discontinued on 12/31/2020. BclI, Art. Examples are BclI (NEB #R0160), (TGATCA) or ScrFI (NEB #R0110) (CCNGG). 1. BclI-HF（星选酶） 货 号 #R3160L-NEB酶试剂 上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。 BclI, recombinant, Article Number: R0160L This product is no longer available. 53 BclI-HF has the same specificity as BclI (NEB #R0160), but it has been engineered for reduced star activity. com Social Media BclI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10187673. Please select an alternative here. 2. The various sticky ends produced by BglI cleavage can be used to reconstitute plasmid and phage genomes and to exchange wild-type and mutant DNA fragments (Burger, K. B852 D-65926 Frankfurt am Main Tel: +49-69/305-23140 E-Mail: info. m36n, cx8pi, uzopfp, ftwfdm, 1xvm, bsufwl, qv5gz, uk27, bono9, oxgp9,